anti hif 1α mab (Novus Biologicals)
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Novus Biologicals
anti hif 1α mab
Anti Hif 1α Mab, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hif+1%CE%B1/HIF-1+alpha+Antibody+(HA111)+-+BSA+Free/pmc13084250-344-22-25
Average 93 stars, based on 8 article reviews
Anti Hif 1α Mab, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hif+1%CE%B1/HIF-1+alpha+Antibody+(HA111)+-+BSA+Free/pmc13084250-344-22-25
Average 93 stars, based on 8 article reviews
anti hif 1α mab - by Bioz Stars,
2026-09
93/100 stars
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Incubation:Article Title: pVHL status in clear cell renal cell carcinoma regulates HIF-α and E-cadherin expression levels and their implications for tumor progression Article Snippet: .. The sections were incubated with primary antibodies at the following dilutions: Article Title: Uremic toxin p-cresyl sulfate enhances the calcification of aortic valvular interstitial cells via klotho/sirtuin-1 signaling Article Snippet: .. The membranes were blocked with 10% non-fat milk in PBS for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against RUNX2 (1:2,000 dilution; cat. no. 12556; Cell Signaling Technology), acetylated-NF-κB (1:2,000 dilution; acetyl-NF-κB; cat. no. 3045; Cell Signaling Technology, Inc.), NF-κB (1:3,000 dilution; cat. no. 8242; Cell Signaling Technology, Inc.), Article Title: Role of IL33/HIF1α/VEGF in intermittent hypoxia-induced lung injury. Article Snippet: Reverse transcription reactions were performed using the TransScript® one-Step Gdna Removal and CDNA Synthesis SuperMIX kit (AT311–03; TRAN, China) according to the manufacturer’s instructions, and Real-time fluorescence quantification in a real-time fluorescence quantitative PCR reaction system (Thermo Fisher Scientific, USA) using the PerfectStartTMGreen QPCR SuperMIX kit (AQ601–02; TRAN, China), the melting curve was analyzed at the end of the reaction, and the data were normalized to quantify the gene expression levels using 2-ΔΔCt. .. 40 mg protein was extracted from lung tissue, 80 μg and 20 μg protein samples were separated by 8 % and 10 % SDS-PAGE gels, respectively, and transferred to a PVDF membrane, which was closed with 5 % skimmed milk powder for 2 h at room temperature, and then incubated at 4 ◦C with Article Title: Uremic toxin p‑cresyl sulfate enhances the calcification of aortic valvular interstitial cells via klotho/sirtuin‑1 signaling. Article Snippet: .. The membranes were blocked with 10% non‐fat milk in PBS for 1 h at room temperature and incubated overnight at 4 ̊C with primary antibodies against RUNX2 (1:2,000 dilution; cat. no. 12556; Cell Signaling Technology), acetylated‐NF‐κB (1:2,000 dilution; acetyl‐NF‐κB; cat. no. 3045; Cell Signaling Technology, Inc.), NF‐κB (1:3,000 dilution; cat. no. 8242; Cell Signaling Technology, Inc.), Chromatin Immunoprecipitation:Article Title: The HIF-2 Transcription Factor Mediates Resistance to Ferroptosis in Pancreatic Cancer Article Snippet: HIF-1α (for WB) , BD Biosciences , Cat# 610959. .. other:Article Title: HIF‐1 Targeting Intervention Renders Protection From Alzheimer's‐Like Pathology in a Humanized Mice Model of HIV Infection Article Snippet: The following reagents and antibodies were used in this study: HIF‐1α siRNA (sc‐35561, Santa Cruz Biotechnology, TX, USA), anti‐human HIV‐1 p24 (clone Kal‐1, M0857, Dako, 1:10), SDS Page:Article Title: Role of IL33/HIF1α/VEGF in intermittent hypoxia-induced lung injury. Article Snippet: Reverse transcription reactions were performed using the TransScript® one-Step Gdna Removal and CDNA Synthesis SuperMIX kit (AT311–03; TRAN, China) according to the manufacturer’s instructions, and Real-time fluorescence quantification in a real-time fluorescence quantitative PCR reaction system (Thermo Fisher Scientific, USA) using the PerfectStartTMGreen QPCR SuperMIX kit (AQ601–02; TRAN, China), the melting curve was analyzed at the end of the reaction, and the data were normalized to quantify the gene expression levels using 2-ΔΔCt. .. 40 mg protein was extracted from lung tissue, 80 μg and 20 μg protein samples were separated by 8 % and 10 % SDS-PAGE gels, respectively, and transferred to a PVDF membrane, which was closed with 5 % skimmed milk powder for 2 h at room temperature, and then incubated at 4 ◦C with Membrane:Article Title: Role of IL33/HIF1α/VEGF in intermittent hypoxia-induced lung injury. Article Snippet: Reverse transcription reactions were performed using the TransScript® one-Step Gdna Removal and CDNA Synthesis SuperMIX kit (AT311–03; TRAN, China) according to the manufacturer’s instructions, and Real-time fluorescence quantification in a real-time fluorescence quantitative PCR reaction system (Thermo Fisher Scientific, USA) using the PerfectStartTMGreen QPCR SuperMIX kit (AQ601–02; TRAN, China), the melting curve was analyzed at the end of the reaction, and the data were normalized to quantify the gene expression levels using 2-ΔΔCt. .. 40 mg protein was extracted from lung tissue, 80 μg and 20 μg protein samples were separated by 8 % and 10 % SDS-PAGE gels, respectively, and transferred to a PVDF membrane, which was closed with 5 % skimmed milk powder for 2 h at room temperature, and then incubated at 4 ◦C with |
